Variant type and patient sex affect molnupiravir efficacy, study finds NatureComms
The following
CoV-2 , VOC alpha , VOC beta , and VOC gamma , BEI cat# NR-54982). VOC delta and VOC omicron were obtained from the Northwestern Reference laboratory and amplified on Calu-3 cells. All viruses were authenticated by whole genome next-generation sequencing prior to use.cells per well the day before infection. Each isolate was diluted in DMEM to achieve a multiplicity of infection of 0.
CoV-2 N and goblet cells, cells were permeabilized for 30 minutes with a 200 µl apical overlay of PBS with 0.1% Triton X-100 and 3% bovine serum albumin , then the overlay was removed and cells were apically incubated with 100 µl of primary antibodies in wash buffer for 1 hour at RT. After three 5-minute 200 µl-washes with wash buffer, cells were incubated with secondary antibodies in wash buffer for 1 hour at RT.
CoV-2 titers and the presence of viral RNA.Two groups of six source ferrets were inoculated intranasally with 1 × 10pfu of VOC alpha, gamma, or omicron. After 12 hours, groups of source ferrets were further divided into two groups, receiving vehicle or molnupiravir treatment administered by oral gavage. At 54 hours after infection, each source ferret was co-housed with uninfected and untreated contacts.
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