A study in MicrobiomeJ underscores how antibiotics impact the fungal microbiome community via disruptions in the bacterial community. This can help understand the gut microbiota equilibrium better to modify medical practices. Read the paper here:
]. Here, we used 19 samples from 7 infants, the same 7 infants before and after receiving amoxicillin because of otitis media as a complication of RSV. Fecal samples were collected before and during amoxicillin treatment and at different timepoints . The samples were immediately frozen at −20 °C and transported frozen at −70 °C until analyzed. The median age of the infants included was 2—4 months .
A 6-week-old female C57BL/6J mice were purchased from Janvier Laboratory and used 1 week after delivery. Animals were kept in humidity- and temperature-controlled rooms under a 12-h light-dark cycle and had access to a chow diet and water ad libitum. All experiments were performed in accordance with the ethics committee “Comite d’Ethique en Experimentation Animale” . Every experiment was repeated at least two times.].
Mice were fasted for 1 h and then subjected to bowel cleansing by oral-gastric gavage with PEG . Four hours later, mice received human feces by oral gastric gavage . Mice were then allowed free access to food and water. FMT was repeated once a week for 3 weeks, before the antibiotic or before and during the bacterial administration. Bowel cleansing was only performed on day 21.SC5314 was used in this study.
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