New gene-editing technique reverses vision loss in mice RockUPress JExpMed
Retinas were dissected out and incubated at 37°C for 20 min in the thermomixer with the activated Papain mix composed of 40 μl Papain , 40 μl Cysteine/EDTA mix , and 320 μl HBSS/Hepes . The cell pellet was collected and treated with benzonase at 37°C for 8 min. After the removal of supernatant, dissociated cells were resuspended in an appropriate volume of DPBS . GFPH&E staining
Retinas were separated from the enucleated eyes and subjected to fixation in 4% paraformaldehyde for 30 min. After dehydration with a series of increasing ethanol concentrations and incubation in xylene, retinas were embedded in paraffin and sectioned at 5 μm thickness with Leica microtome. Following the deparaffinization and rehydration, the slides with retinal sections were stained with H&E and mounted with Canada balsam . Images were captured with an Olympus IX83 microscope.
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Very low-calorie ketogenic diet (VLCKD): an antihypertensive nutritional approach - Journal of Translational MedicineBackground Obesity is accompanied by hormonal, inflammatory and endothelial alterations. These alterations induce a stimulation of several other mechanisms that contribute to the hypertensive state and to increase the cardiovascular morbidity. This pilot, open - label, single- center, prospective clinical trial aimed to evaluate the effect of very low- calorie ketogenic diet (VLCKD) on blood pressure (BP) in women with of obesity and hypertension. Methods A total of 137 women, who met the inclusion criteria and accepted to adhere to VLCKD, were consecutively enrolled. Assessment of anthropometric parameters (weight, height, and waist circumference), body composition (through bioelectrical impedance analysis), systolic (SBP) and diastolic blood pressure (DBP) and blood sample collection were carried out at baseline and after 45 days of the active phase of VLCKD. Results After VLCKD all the women experienced a significant reduction in body weight and an overall improvement of body composition parameters. In addition, high sensitivity C reactive protein (hs- CRP) levels were significantly diminished (p | 0.001), while phase angle (PhA) increased by almost 9% (p | 0.001). Interestingly, both SBP and DBP were significantly improved (-12.89% and − 10.77%, respectively; p | 0.001). At baseline, SBP and DBP showed statistically significant correlations with body mass index (BMI), waist circumference, hs-CRP levels, PhA, total body water (TBW), extracellular water (ECW), Na / K ratio, and fat mass. Even after VLCKD, all correlations among SBP and DBP with the study variables were statistically significant, except for the association between DBP and Na / K ratio. Changes (%) in both SBP and DBP were associated with ∆BMI%, ∆PhA% and ∆hs- CRP levels (p | 0.001). In addition, only ∆SBP% was associated with ∆waist circumference (p = 0.017), ∆TBW (p = 0.017), and ∆fat mass (p | 0.001); while only ∆DBP% was associated with ∆ECW (p = 0.018), and ∆Na / K ratio (p = 0.048). After adju
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Martin Lewis shares 'secret' code that could help you save money on medicineMARTIN Lewis has shared a little-known “secret” code that could save you money when buying over-the-counter medicines. The money saving expert, 50, revealed the tip on a recent episode of his Money…
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100 Gecs' 10,000 Gecs is stupid and brilliant - the peak of a panic attack★★★★★ This album is the musical equivalent of a strobe light. It is a sugar rush, the caffeine jitters, the peak of a panic attack. 10,000 gecs is chaos and it is stupid and it is brilliant 🖋️ katiesol
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From blurred vision to forgetfulness - the signs of a brain tumour you must knowBRAIN tumours can cause a host of different symptoms, depending on their position. Most commonly, you might experience headaches, sickness, or seizures if you have one. Each year around 12,300 case…
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Dementia risk is reduced by a Mediterranean dietDementia risk is reduced by a Mediterranean diet Dementia Diet Genetic PublicHealth UKBiobank MedDiet MediterraneanDiet BMCMedicine UniofNewcastle UniofExeter EdinburghUni Deakin orebrouni Cambridge_Uni ohiou
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Artificial pancreas improves blood sugar control for kids ages 2-6, study findsAn artificial pancreas originally developed at the University of Virginia Center for Diabetes Technology improves blood sugar control in children ages 2 to 6 with type 1 diabetes, according to a new study. Details of the clinical study and its findings have been published in the New England Journal of Medicine.
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