New diagnostic test is 1,000 times more sensitive than conventional tests wustl natBME
for plasmonic fluors). The optimum number of nanolabels for colorimetric AuNPs-LFA and p-LFA, and fluorometric p-LFA was determined by subtracting the background signal from the test spot signal.biotinylated BSA onto the nitrocellulose membrane. The LFA strips were assembled as described above.
SARS-CoV-2 S1 antibody ELISA was carried out according to the following procedure. Microtitre wells in duplicates were coated with 100 µl of 5 µg ml recombinant SARS-CoV-2 S1 protein via overnight incubation at room temperature. For blocking, 300 µl reagent diluent was added to the wells for a minimum of 1 h.
To prepare nanolabels for the test line, 1–3 µl of biotinylated SARS-CoV-2 N protein or human IL-6 detection antibody of 1 mg mlconcentration was added to 1 ml streptavidin functionalized plasmonic fluors of extinction 2. After 30 min incubation, 100 µl of 10% BSA in 1× PBS was added to this antibody-conjugated plasmonic-fluor solution.
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