Nature research paper: Two defence systems eliminate plasmids from seventh pandemic Vibrio cholerae
Extended Data Fig. 2 Conservation of the plasmid stability phenotype dependent on VC1771-70 and VC0492-90., pSa5Y-Kan stability in representative wild-type 7PET strains and their ΔVC1771 derivatives after growth for approx. 50 generations without antibiotic selection., Comparison of VC1769-1772 transcript levels between strains A1552, classical O395 and ATCC25872 in exponentially growing cells as determined by quantitative reverse transcription PCR .
, pSa5Y-Amp stability in strain A1552 encoding a classical variant of VC1771, in the presence and absence of VC0490-93., Retention of plasmids with various origins of replication in strain A1552 and its ΔVC1770 and ΔVC0490 derivatives. The different origins of replication were cloned into a neutral plasmid backbone containing a conditional origin of replication inactive in the tested strains. Plasmid stability in was evaluated after growth for approx. 50 generations without antibiotic selection.
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