The function and evolution of a genetic switch controlling sexually dimorphic eye differentiation in honeybees NatureComms
sp. hosts at 25 °C. Male and female wasps were separated based on the sex-specific forewing size before eclosion. Male-only offspring were generated by offering hosts to virgin females. To produce female offspring, a virgin female was paired with a single male and allowed to mate for one day. Two hosts per day were provided to individual females to initiate oviposition.Genomic DNA was isolated from honeybee L1 larva, queen leg or pupal hindleg tissue with the innuPREP DNA Mini kit .
Phusion™ High-Fidelity DNA Polymerase was used for RT-PCR if the amplicons were to be subsequently sequenced. Otherwise, GoTaq® G2 Flexi DNA Polymerase was used. PCR was performed with a standard temperature profile . For the semiquantitative RT-PCR, the amount of template and the number of cycles were adjusted according to reference gene) and
to exclude saturation and allow adjustment across samples. The employed oligonucleotides with their sequences are listed in the Supplementary DataSequence analyses
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