Nature research paper: The cellular coding of temperature in the mammalian cortex
Electrophysiological recordings for measuring the impact of optogenetic inhibition on thermal responses were carried out in VGAT-ChR2 mice implanted with a head post. Mice were habituated for head and paw fixation. On the day of the recording, the mouse was briefly anaesthetized and a craniotomy of about 2 mm diameter was carried out over the pIC. The dura was removed in the ventral part of the craniotomy and the brain was covered with Kwik-Cast silicon .
We carried out 7 recordings in 4 mice using 32-channel silicon probes in either a linear or four-shank ‘Buzsaki32’ configuration. In some experiments, the tips of the probe were covered with DiI . The probe was inserted orthogonal to the pIC with a micromanipulator at 1–2 µm s. Recordings were acquired at 30 kHz with a Neuralynx system . The extracellular recordings were spike sorted using Kilosort .
Two-second-long thermal stimuli of 10 °C cooling and warming were interleaved randomly and delivered every 30 s. The LED fibre was tilted slightly to accommodate the silicone probe. The LED was on for the entire duration of the thermal stimulus and delivered at 20 Hz, 50% duty cycle with a power of 12.5 mW .To label the thermal cortical representation, animals were deeply anaesthetized by intraperitoneal injection of ketamine and xylazine.
Brasil Últimas Notícias, Brasil Manchetes
Similar News:Você também pode ler notícias semelhantes a esta que coletamos de outras fontes de notícias.
Net loss of biomass predicted for tropical biomes in a changing climate - Nature Climate ChangePrecipitation and temperature affect biomass and carbon storage in the tropics. This study shows that warming-driven contraction of humid regions and expansion of areas with dry periods could double carbon losses, with about one-third associated with decline of humid areas in South America.
Consulte Mais informação »
2022 – toughing out the trough - Nature BiotechnologyInvestor faith in biotech stocks fell further in 2022 after a flat performance for the sector in the previous year, according to our latest Data Page. Biotech IPOs raised less than one-eighth of the money they did in 2021
Consulte Mais informação »
Universal logic with encoded spin qubits in silicon - NatureNature research paper: Universal logic with encoded spin qubits in silicon
Consulte Mais informação »
7 types of rainbows: Nature's mesmerizing optical phenomenaThere are several types of rainbows – not just the multicolored bright arc frequently observed when the sun comes out on a rainy day.
Consulte Mais informação »
Fighting the Louisiana Law That Makes Sex Work a “Crime Against Nature”In the short documentary “CANS Can’t Stand,” by Matt Nadel and Megan Plotka, activists work to overturn a baldly prejudiced law aimed at L.G.B.T.Q. people.
Consulte Mais informação »
A quantum-bit encoding converter - Nature PhotonicsA conversion of quantum information between single-photon and cat-state qubits is demonstrated by teleportation using optical hybrid entanglement. The classical limit of conversion is exceeded over the full Bloch sphere, with an average fidelity above 79%.
Consulte Mais informação »