itemprop=description content=Scientists testing a new method of sequencing single cells have unexpectedly changed our understanding of the rules of genetics.
Reviewed by Megan Craig, M.Sc.Oct 6 2023 Scientists testing a new method of sequencing single cells have unexpectedly changed our understanding of the rules of genetics.
The work was intended to test a DNA sequencing pipeline to work with very small amounts of DNA, such as DNA from a single cell. Dr McGowan was working with a team of scientists at the Earlham Institute and with Professor Thomas Richards' group at the University of Oxford. "The definition of a protist is loose - essentially it is any eukaryotic organism which is not an animal, plant, or fungus," said Dr McGowan. "This is obviously very general, and that's because protists are an extremely variable group.
Ciliates are hotspots for genetic code changes, including reassignment of one or more stop codons - the codons TAA, TAG, and TGA. In virtually all organisms, these three stop codons are used to signal the end of a gene. DNA is like a blueprint of a building. It does not do anything in and of itself – it provides instructions for work to be done. In order for a gene to have an impact, the blueprint must be "read" and then built into a molecule which has a physical effect.
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