A study published in Cancer Cell International finds that spalt-like protein 4 plays a critical role in gastric cancer angiogenesis by modulating vascular endothelial growth factor expression.
In this study, we investigated the biological roles and mechanisms of SALL4 in the pathogenesis of gastric cancer. We found that the upregulation of SALL4 in STAD was positively correlated with tumor progression. Also, we found that SALL4-B downregulation inhibited, while overexpression enhanced, the pro-angiogenic effect of gastric cancer cells. In addition, we also discovered that SALL4 promoted angiogenesis via the regulation of the VEGF gene.
green on a Bio-Rad CFX96 system. The 2method was used to determine mRNA fold changes. β-actin served as a normalization control. The primer sequences are listed in .The transfected and treated cells were cultured in RPMI-1640 containing 10% FBS and plated on a 6-well plate . The cells were washed after 24 h and changed from normal growth medium to serum-free medium. After 48 h of incubation, cell culture supernatants were collected and centrifuged at 2000 rpm to remove cell debris.
Cs were resuspended in a serum-free medium and placed in the upper chamber . The lower chamber was then incubated with the indicated CMs for 24 h. Penetrated cells were stained with crystal violet, and images were taken under a microscope to count the cells.The level of VEGF-A, B, and C in si-SALL4-B, P-SALL4-B, CRISPR/Cas9-KO-SALL4, MGC-803-Thalidomide or engineered exosomes cells’ conditioned medium were determined using an ELISA kit according to the manufacturer’s protocol .
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