Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy - Nature Biomedical Engineering

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Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy - Nature Biomedical Engineering
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Expansion revealing (ExR) enables high resolution microscopic visualization of molecules and cellular structures in synapses of mice with Alzheimer's-related plaques NBThighlight

We thank T. Biederer, C. Zhang and Y. Liu for antibody advice, S. Alon and K. Piatkevich for trainings and discussions; B. Kang for decrowding analysis advice; and D. Goodwin for instructions and scripts for manual image registration. D.S. acknowledges funding from NIH K99/R00 Pathway to independence Award ; M.E.S was supported by the National Science Foundation Graduate Research Fellowship under Grant No. 1745302 and the MathWorks Science Fellowship; A.-H.T. acknowledges funding from NARSAD; E.

acknowledges funding from the Ludwig Family Foundation, the Open Philanthropy Project, John Doerr, Lisa Yang and the Tan-Yang Center for Autism Research at MIT, the US Army Research Laboratory and the US Army Research Office under contract/grant number W911NF1510548; Charles Hieken, Tom Stocky, Kathleen Octavio, Lore McGovern, Good Ventures; NIH Director’s Pioneer Award 1DP1NS087724, NIH R01MH110932, NIH R01EB024261, NIH U24NS109113, NIH R37MH08004613, NIH 1RM1HG008525, NIH 1R01AG070831, NIH...

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