Study investigates effects of isoeugenol on fish unibt CommsBio
= 10 fish each. One group was exposed to isoeugenol [20 mg L] for 30 min. Then the fish of the exposure group were transferred to a tank containing fresh water and no anesthetic agent. After the transfer, the fish were observed for 30 min and a light flash as used in the electrophysiological experiments was presented at least once per minute. When a fish did not respond to a light flash, a second flash could be given within 30 to 60 s.
Although it also may be of interest whether the reversibility of the effects on vision differs when higher doses are administered, we did not test higher concentrations: At higher concentrations breathing slows down too much in the experimental fish , so the fish might suffer at the high concentrations over the extended exposure time. Because vision was blocked at much lower concentration, we used these lower concentrations to study if vision comes back.
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