Recombinant SARS-CoV-2 harboring double reporters for COVID-19 surveillance SARSCoV2 Coronavirus Disease COVID GeorgiaStateU txbiomed UABNews biorxivpreprint
Study: Monitoring SARS-CoV-2 infection using a double reporter-expressing virus. Image Credit: ktsdesign / Shutterstock
About the study In the present research, the authors, for the first time, developed a replication-competent rSARS-CoV-2 that expresses both luciferase and fluorescent reporter genes to circumvent the drawbacks of using a single reporter gene. Generation of a bireporter rSARS-CoV-2 expressing mCherry and Nluc . A) Schematic representation of the rSARS-CoV-2/mCherry-Nluc viral genome: SARS-CoV-2 structural, non-structural, and accessory open reading frame proteins are indicated in white boxes. mCherry , Nluc and the PTV-1 2A autoproteolytic sequence were inserted in front of the viral N protein.
The authors discovered that the half-maximal effective concentration of antivirals and the 50% neutralizing titer of NAbs achieved in bireporter-based microneutralization experiments using either fluorescence or luciferase signal were identical to those obtained with rSARS-CoV-2/WT, rSARS-CoV-2/Nluc or rSARS-CoV-2/mCherry reporter genes, and those documented in the prior literature.
Conclusions On the whole, the team created an rSARS-CoV-2 that expresses the luciferase and fluorescent genes, namely rSARS-CoV-2/mCherry-Nluc, in the present study. They showed that rSARS-CoV-2/mCherry-Nluc could be used to research SARS-CoV-2 biology in vivo and or in vitro, such as characterization and identification of NAbs and or antivirals. The scientists depict SARS-CoV-2 infection and transmission via IVIS using rodent models.
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