RBD-NTD-subunit protein vaccine provides a promising booster vaccination strategy capable of protecting against known SARS-CoV-2 variants WellingtonUni otago UniMelb protein vaccine booster vaccination SARSCoV2
By Bhavana KunkalikarFeb 22 2023Reviewed by Danielle Ellis, B.Sc. In a recent study published in iScience, researchers assessed the impact of severe acute respiratory syndrome coronavirus 2 spike N-terminal domain incorporation into the receptor-binding domain protein vaccine.
Unfortunately, the RBD by itself is a weak immunogen, failing to produce substantial amounts of NAbs in most cases. Multimerization of the RBD can enhance its immunogenicity, resulting in enhanced B cell receptor crosslinking and enhanced antigen-presenting cell phagocytosis. To assess the effect of design architecture on the expression, binding, and stability of angiotensin-converting enzyme-2 , the study generated controls comprising an RBD monomer as well as an RBD tandem dimer belonging to each strain and an RBD-NTD heterodimer.
Antibodies eBook Compilation of the top interviews, articles, and news in the last year. Download a free copy On day 14, a cohort of convalescent mice was established utilizing a sub-lethal dosage of 102 TCID50 SARS-CoV-2 Wuhan strain. On day 35, the mice were inoculated with 1 x 104 TCID50 with the Delta variant, and their body weight, as well as mortality, were recorded.
According to affinity calculations, dimerizing the RBD quadrupled its binding affinity to ACE2. However, the Kd observed for all other constructs evaluated in this study was relatively similar at less than 2 nM, indicating that the presence of the NTD domain in the VAANZ-W RRN protein did not affect ACE2 interaction. All constructs were appropriately folded, and the presence of the NTD at the RBD dimer C terminus reported no effect on the RBD/ACE2 interaction.
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