Q-LAAD: a dynamic, cost-effective rapid antigen test for detection of SARS-CoV-2 medrxivpreprint BoiseState SARSCoV2 COVID19 coronavirus covid
By Dr. Chinta SidharthanSep 28 2022Reviewed by Danielle Ellis, B.Sc. In a recent study posted to the medRxiv* preprint server, researchers developed a high-throughput, rapid antigen severe acute respiratory syndrome coronavirus 2 spike protein detection test using allonamers, a new type of aptamers that carry out allosteric regulation instead of catalytic activity.
Antigen-detecting rapid diagnostic tests target viral spike and nucleocapsid proteins in the respiratory tract and are a more suitable option for early detection of COVID-19. However, their dependence on highly specific monoclonal antibodies limits their widespread usage. A DNA allonamer was produced using the systematic evolution of ligands by exponential enrichment . This allonamer consisted of two binding domains with a linker region. The binding of the SARS-CoV-2 spike protein to one domain would result in a conformational change in the other binding domain, allowing it to connect to a fluorescent reporter molecule.
When Q-LAAD was tested against the alpha, beta, gamma, and delta SARS-CoV-2 variants, the fluorescence signals from detecting gamma and delta spike proteins were significantly higher than the nucleocapsid control, while the signals from binding to alpha and beta spike proteins showed a five-fold change, although it was not significant.
Brasil Últimas Notícias, Brasil Manchetes
Similar News:Você também pode ler notícias semelhantes a esta que coletamos de outras fontes de notícias.
What are the neuropsychiatric effects of SARS-CoV-2 infection?What are the neuropsychiatric effects of SARS-CoV-2 infection? medrxivpreprint UMmedschool neuropsychiatric neurology neuroscience psychiatry infection COVID19 coronavirus covid SARSCoV2
Consulte Mais informação »
Study investigates long-range RNA-RNA interactions of SARS-CoV-2Researchers explored RRIs [long-range ribonucleic acid (RNA)-RNA interactions] in SARS-CoV-2 variants of concern genomes to assess evolutionary changes in SARS-CoV-2.
Consulte Mais informação »
Systemic inflammation triggered by SARS-CoV-2 found to cause human testicular injuryIn a new study, a team of researchers demonstrated that testicular injury in humans is not due to direct SARS-CoV-2 infection but more likely is an indirect effect of exposure to systemic inflammation or SARS-CoV-2 antigens.
Consulte Mais informação »
Study characterizes immunological signature of SARS-CoV-2 infection in childrenA recent study has demonstrated that the clinical severity of COVID-19 in children depends on the dynamics of immune response induced by SARS-CoV-2 infection.
Consulte Mais informação »
Rational treatment strategies for SARS-CoV-2 derived from human pluripotent stem cells modelsIn a recent study posted to the bioRxiv* preprint server, researchers in Australia explored the molecular mechanisms of the pulmonary and cardiac complications caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection using human stem-cell-derived cardiac and lung cells.
Consulte Mais informação »
Observing SARS-CoV-2 Omicron reinfections with different sub-variantsObserving SARS-CoV-2 Omicron reinfections with different sub-variants SARSCoV2 COVID19 Omicron Reinfection CDCgov
Consulte Mais informação »