Precise transcript targeting by CRISPR-Csm complexes
Cell viability and proliferation assay
The WST-1 assay was used to quantify cell viability and proliferation. Cells transfected with Csm, Cas13 or shRNA plasmids were grown in 96-well plates until the indicated timepoints, incubated with WST-1 reagent at 37 °C for 1 h as per manufacturer’s instructions, and absorbance measured using a Cytation five microplate reader at 450 nm with 600 nm reference.
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