What makes mice fat, but not rats? Suppressing NMU, study finds SciReports
. Pre-estrous F344 female rats were mated with male rats overnight and then copulation plugs were visually inspected the following morning and used for electroporation experiments. Electroporation experiments were operated on anesthetized female rats .
Rats were maintained as two animals per cage after weaning. Body weight was measured weekly from 4 to 24 weeks of age during normal chow feeding and from 4 to 15 weeks of age during high fat chow feeding .Rats were maintained in an individual cage from 8 weeks of age and with food and water provided ad libitum. The amounts of food and water intake were measured weekly from 9 to 24 weeks of age to calculate average daily amounts.
The specificity for the newly raised antibody was confirmed by western blot analysis to recognize synthetic rat NMU peptide . Synthetic rat NMU peptide was subjected to 16% SDS-PAGE and transferred onto PVDF blotting membranes. The membranes were blocked with 5% dry skim milk in Tris-buffered saline containing 0.1% Tween-20 for 1 h. Immunoblotting was performed with the anti-NMU antibody, followed by a horseradish peroxidase-conjugated secondary antibody .
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