New imaging technique can capture entire planttissues in 3D salkinstitute NaturePlants
root tips were immersed in FAA for 1 h at room temperature. RNase-free water was used throughout the entire protocol. Samples were then dehydrated in a series of 10-min washes once in 70% ethanol, once in 90% ethanol and twice in 100% ethanol, followed by two 10-min washes in 100% methanol, and then were stored in 100% methanol at −20 °C overnight. The next day, samples were rehydrated in a series of 5-min washes in 75% , 50% and 25% methanol in DPBS-T at room temperature.
probes was heated at 90 °C for 5 min and cooled at room temperature. Samples were incubated in hybridization buffer in a 40 °C humidified oven overnight. After hybridization, samples were washed twice in DPBS-TR and once in 4× SSC in DPBS-TR for 30 min at 37 °C and rinsed with DPBS-TR at room temperature. Samples were then incubated in a T4 DNA ligation mixture at room temperature overnight.
probes for UBQ10 were used .The cost of PHYTOMap experiments is approximately US$80 for a 28-gene experiment and US$230 for a 96-gene experiment (Supplementary Fig.
Brasil Últimas Notícias, Brasil Manchetes
Similar News:Você também pode ler notícias semelhantes a esta que coletamos de outras fontes de notícias.
Interchange reconnection as the source of the fast solar wind within coronal holes - NatureMeasurements of fast solar wind streams from the Parker Solar Probe spacecraft provide strong evidence for the interchange reconnection mechanism being responsible for accelerating the fast solar wind.
Consulte Mais informação »
Owner of Byron ranch charged with illegally growing 458,353 marijuana plantsThe suspect, an Alameda resident, was charged with three misdemeanors for allegedly growing hundreds of thousands of marijuana plants under the guise of building a hemp business.
Consulte Mais informação »
This 'Stunning' 14-Sided New Shape May Deepen How We Understand 'Order in Nature'David Smith, a retired print technician from the north of England, was pursuing his hobby of looking for interesting shapes when he stumbled onto one unlike any other in November.
Consulte Mais informação »
Health system-scale language models are all-purpose prediction engines - NatureA clinical language model trained on unstructured clinical notes from the electronic health record enhances prediction of clinical and operational events.
Consulte Mais informação »
Robot gardener grows plants as well as humans do but uses less waterA vegetable-growing trial has pitted expert human gardeners against an AI-powered robot – both produced comparable crops, but the robot used about 40 per cent less water
Consulte Mais informação »
Dendrite initiation and propagation in lithium metal solid-state batteries - NatureAnalysis of dendrite initiation, owing to filling of pores with lithium by means of microcracks, and propagation, caused by wedge opening, shows that there are two separate processes during dendrite failure of lithium metal solid-state batteries.
Consulte Mais informação »