The growth of the 'optic cups' mirrored the process that occurs in the embryo.
on brain organoids that they had made in their lab. The growth of eye structures in the lab mirrored the one that occurs in the embryo and will help us understand how eyes develop. too. However, studying the human brain is not that easy. It is locked up inside the skull for its active period and available for analysis only after its stops to function. Most brain development also occurs quite early in life, leaving little scope for an investigation into its development and workings.
Neuroscientist Jay Gopalakrishnan at University Hospital Dusseldorf and his team of researchers are interested in studying diseases of the eye. To understand, how these diseases occur in the first place, they need to understand the process of eye development. Other researchers had previously, used iPSCs to develop optic cups, structures that lead to the development of the retina - the light-sensitive layer of cells in the eye.
The retina plays an important role in the eye. It converts the light it receives into neural signals that it transmits to the brain using the optical nerve. The brain then analyses the signals, which in common terms is called 'sight'. Since the retina works closely with the brain, Gopalkrishnan and his team decided to grow the optic cups on brain cells, that were sourced from iPSCs.
Using samples from four iPSC donors, the team first made the brain organoids and then modified its growth media to induce the formation of optic cups. and demonstrated connections to the brain cells. During embryonic development, the retinal cells reach out to the brain but this was never demonstrated in the lab before, until this work. The optic cups appeared as early as 30 days into the brain development and became distinctly visible by 50 days.
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