Study reveals intensive grassland management hampers recovery of soil food webs from drought AdmissionsUoM NatureComms
N addition. The holes created were filled back with sand to minimise gas exchange from the exposed surface and disturbance. Four supplementary cores outside the plots were taken per field to determine theA portion of the intact sample was placed on Tullgren funnels over 7 days to extract mesofauna, collected in 70% ethanol and stored until further analysis. From the remaining sample, plant shoots were collected, and roots were separated from the soil by handpicking, and washed.
Soil microbial communities were characterised by the extraction of the phospholipid fatty acids , according to Buyer and Sasser methodC signatures of individual PLFAs and their quantification were analysed by GC–C–IRMS using a GC Trace Ultra with combustion column attached via a GC Combustion III to a Delta V Advantage IRMS . The internal standard 19:0 phosphati‐ dylcholine added at the beginning of the extraction procedure was used for calculating concentrations.
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