Technology that builds on mRNA-based COVID19 vaccines may help treat influenza and some cancers, according to new research in ScienceAdvances.
). Briefly, a lipid solution of DSPE-PEG and DSPE-PEG2000-maleimide at 4:1 molar ratio was dried under nitrogen and placed in a vacuum chamber for 2 hours to form a thin film. Lipids were rehydrated in PBS at 6.4 mg/ml in a 60°C water bath for 15 min and sonicated in an ultrasonic bath for 5 min.
The sizes of APNs in PBS were measured by dynamic light scattering with Malvern Nano ZS Zetasizer . Final lipid concentration was quantified using a phospholipid assay kit . The concentration of conjugated pMHCI was determined using a BCA assay kit . The mRNA encapsulation efficiency was quantified by Quant-iT RiboGreen RNA assay as previously described .
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