Breakthrough in plantbreeding: Grafting and mobile CRISPR for genome editing in plants NatureBiotech
. The disadvantages of these systems are that many crop plants and fruit trees are inaccessible or that they are difficult to be transiently infected or transformed. For example, in the previous methods the edited plant material still contains thegene and/or remains contaminated by transgenic bacteria or viruses.
Here we present an alternative, rapid method for producing heritable genome edits induced by graft-mobile TLS fusion transcripts. The presented grafting system does not need elimination of gRNA ortransgenes and regeneration of plants from tissues or protoplasts and, thus, the desired mutation can be produced and used after the first generation.
The gene editing method presented here, despite the required production of a transgenic rootstock line, is not more time consuming than alternative transgene-free genome editing methods. Gene editing by grafting confers an advantage as there is no requirement for multiple additional generations to remove the transgene or regeneration of plants from transfected protoplasts.
As mentioned above, the introduction of gene edits by grafting would greatly simplify the production of mutant plants where transformation is difficult, impossible or undesirable. The wild-type scion cultivar grafted onto a given transgenic rootstock donor can belong to a very distant plant family. For example,. can easily be used as rootstocks for a very wide range of quite distantly related species including tomato, carrots, soybean, and onions.
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