Understanding how Aspergillus fumigatus shapes the lungs NatureComms
. Spontaneously expectorated sputum was collected during visits to the Cystic Fibrosis Center at the University Hospital Heidelberg and frozen in liquid nitrogen on the day of visit. Sputum samples were collected from 40 cystic fibrosis patients before and after they had positivecolonization. Samples were frozen within 24 h after reception at the microbiology department. The cohort for 80 total samples was 15 females and 25 males aged 23.6 ± 4.96 years beforecolonization.
DNA fragments were end-polished, A-tailed, and ligated with full-length adapters for Illumina sequencing, followed by PCR amplification with P5 and indexed P7 oligos. PCR products for final construction of libraries were purified with the AMPure XP system . Libraries were checked for size distribution with an Agilent 2100 Bioanalyzer , and quantified by real-time PCR to ensure an amount of at least 3 nM). Qualified libraries were inserted into Illumina sequencers .
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A day-to-day management model improves patient compliance to treatment for Helicobacter pylori infection: a prospective, randomized controlled study - Gut PathogensBackground The day-to-day (DTD) management model encourages patients to actively participate in their healthcare by setting goals. We determined the effectiveness of the DTD model in the treatment of Helicobacter pylori (H. pylori) infection, as compared with conventional outpatient education (OE). Methods We randomized 254 H. pylori-positive patients into a DTD group (127 patients) and an OE group (127 patients) prior to primary treatment with 14-day bismuth-containing quadruple therapy, including esomeprazole, amoxicillin, and clarithromycin. Both groups received consistent medication instructions. Patients in the DTD group recorded daily attendance after completing their daily medication plan from day 1 to day 14. The medication compliance, follow-up compliance, H. pylori eradication rates, and adverse events (AEs) were evaluated. Results In the modified intention-to-treat (MITT) and per-protocol (PP) analyses, the DTD group showed significantly higher medication compliance than the OE group (P = 0.001 and P = 0.031, respectively). Both the MITT and PP analyses showed significant differences in follow-up compliance (P | 0.001 and P = 0.003, respectively) and timing of the review urea breath test (P | 0.001 and P = 0.001, respectively) between the two groups. However, no significant differences were observed in the H. pylori eradication rates (95.8% vs. 93.8%, P = 0.529) in the PP analysis, or AEs incidence (25.4% vs. 28.3%, P = 0.603) between the two groups. Conclusion This study demonstrated the novel application of the DTD model in the treatment of H. pylori infection, which enabled patients to develop habitual medication-taking behaviors without physician intervention.
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