Wild fish thrive despite 'hopeless monster' mutations, according to study sumedicine NatureEcoEvo
cluster and scored in 211 fish from Louisbourg Fortress and 121 fish from Tidnish River 3 .. A 20 µl PCR reaction mixture contained 2× Master Mix , 0.5 µM 6FAM M13 forward universal primer, 0.125 µM forward primer, 0.5 µM reverse primer and 10 ng genomic DNA. The PCR programme was 94 °C , 30 cycles at 94 °C /58 °C /72 °C , 8 cycles at 94 °C /53 °C /72 °C and a final extension at 72 °C for 10 min.
Indel and SNP markers were scored using PCR with 2× Master Mix, 0.5 µM forward primer, 0.5 µM reverse primer and 10 ng genomic DNA. The PCR programme was 95 °C , 35 cycles at 95 °C /54 °C /72 °C and a final extension at 72 °C for 10 min.
. For indel or SNP markers, a chi-squared test was performed in R v.3.6.1. Associations between residual spine lengths and genotypes were quantified using an analysis of variance performed in R.Whole-genome sequencing using 10X Genomics Chromium-linked read technology was performed on twofrom the Louisbourg Fortress population . Genomic DNA was extracted from brains and prepared using the QIAGEN MagAttract HMW DNA kit. Linked read data from each fish were assembled using Supernova v.2.1.
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