Nature research paper: Cohesin-mediated loop anchors confine the locations of human replication origins
Treatment and sample collection timeline of HCT116 RAD21-mAID and HCT116 WAPL-mAID2 cells for high-resolution 16-fraction Repli-seq.HCT116 WAPL-mAID2 cells in asynchronous cultures and immediately after mitotic shake-off conditions,
Western blot of RAD21 protein in HCT116 RAD21-mAID cells for untreated control and timepoints after auxin treatment post mitotic shake off. Ponceau S stain for total protein. Blot run on one set of samples. Total IP efficiency for each of 16 S phase fractions of high-resolution 16-fraction Repli-seq for HCT116 WT, HCT116 RAD21-mAID KD, and HCT116 WAPL-mAID2 KD cells.
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