Chemoproteomic identification of CO2-dependent lysine carboxylation in proteins - Nature Chemical Biology

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Chemoproteomic identification of CO2-dependent lysine carboxylation in proteins - Nature Chemical Biology
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analogues. Data represent mean values ± SD from n = 4 independent experiments., Far-UV CD spectra of OXA-48 pre-incubated in the presence of 50 mM NaCl or 50 mM KOCN. Data represent mean values ± SD from n = 4 independent experiments., Activity assays on samples from taken immediately before and after CD . OXA-48 remains inactivated throughout the CD experiment. Data represent means ± SD from n = 3 independent experiments.

Samples were centrifuged to remove insoluble aggregates prior to immunoblotting. This immunoblot was performed once., Immunoblot competition assays for WT and K73A OXA-48. The image shown in f is a representative image from three independent experiments., Densitometry analysis of select bands from . The chart displays mean ± SD from two experimental replicates with< 0.01. The indicated significant, Crystallographic evidence for hCit73 OXA-48.

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