Aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4, finds a study published in Cancer Cell International.
The CRC cells were plated in 96-well plate . Aspirin and chemotherapy agents were employed to treat CRC cells. At the end of the experiment, CCK8 solution was added in each wells for 1 h and then cell viability was detected at 450 nm by microplate reader.For sphere formation assay, 1000 cells were seeded in Ultra Low Attachment 6-well plates and cultured in DMEM/F12 supplemented with B27, N-2, 20 ng/ml EGF and 20 ng/ml bFGF .
1% Tween-20 for 1 h at room temperature and then incubated with primary antibodies overnight at 4 °C. And then, the membrane was washed with 1 × TBS/0.1% Tween-20 before incubated with the secondary antibody for 1 h at room temperature. Immunoblots were performed by using the BeyoECL Plus substrate system , followed by washing with 1 × TBS/0.1% Tween-20.Small interfering RNA was added according to the manufacturer’s instructions.
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Frontiers | Spatiotemporal local and abscopal cell death and immune responses to histotripsy focused ultrasound tumor ablationHistotripsy is a novel focused ultrasound tumor ablation modality with potent immunostimulatory effects. To measure the spatiotemporal kinetics of local and abscopal responses to histotripsy, C57BL/6 mice bearing bilateral flank B16 melanoma or Hepa1-6 hepatocellular carcinoma tumors were treated with unilateral sham or partial histotripsy. Treated and contralateral untreated (abscopal) tumors were analyzed using multicolor immunofluorescence, digital spatial profiling, RNA sequencing (RNASeq), and flow cytometry. Unilateral histotripsy triggered abscopal tumor growth inhibition. Within the ablation zone, early high mobility group box protein 1 (HMGB1) release and necroptosis were accompanied by immunogenic cell death transcriptional responses in tumor cells and innate immune activation transcriptional responses in infiltrating myeloid and natural killer (NK) cells. Delayed CD8+ T cell intratumoral infiltration was spatiotemporally aligned with cancer cell features of ferroptosis; this effect was enhanced by CTLA-4 blockade and recapitulated in vitro when tumor-draining lymph node CD8+ T cells were co-cultured with tumor cells. Inoculation with cell-free tumor fractions generated by histotripsy but not radiation or freeze/thaw conferred partial protection from tumor challenge. We propose that histotripsy may evoke local necroptotic immunogenic cell death, priming systemic adaptive immune responses and abscopal ferroptotic cancer cell death.
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Black women in England more likely to be diagnosed with late-stage cancerCancer Research is calling for the government to increase cancer awareness and screenings for black communities
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Single-cell T cell receptor sequencing of paired human atherosclerotic plaques and blood reveals autoimmune-like features of expanded effector T cells - Nature Cardiovascular ResearchDepuydt and Schaftenaar et al. profile the T cell clonality in patients with atherosclerosis by performing single-cell T cell receptor sequencing on carotid artery plaques and matched peripheral blood mononuclear cell samples. The analyses showed plaque-specific clonal expansion in effector CD4+ T cells, expressing genes indicative of exposure to activating antigens, thus suggesting that atherosclerosis has an autoimmune component driven by autoreactive CD4+ T cells.
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'Spleen-on-a-chip' yields insight into sickle cell diseaseEvery day, billions of red blood cells pass through the spleen, an organ that is responsible for filtering out old or damaged blood cells. This task is made more difficult when the blood cells are misshapen, as they are in patients with sickle cell disease, which affects millions of people throughout the world. Sickled blood cells can clog the spleen's filters, leading to a potentially life-threatening situation.
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Andrew Tate: A timeline from Twitter rows to a Romanian cellAndrew Tate is being held in Romania on suspicion of rape and human trafficking. Between police seizing his luxury cars, court appeals and a spat with Greta Thunberg, you might have missed exactly what happened when. Here's what led up to his arrest and what has happened since.
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Single-cell analysis reveals prognostic fibroblast subpopulations linked to molecular and immunological subtypes of lung cancer - Nature CommunicationsFibroblast heterogeneity is a prominent but poorly understood feature of solid tumours. Here three major fibroblast subpopulations in non-small cell lung cancer are identified and characterised through single cell RNA-sequencing, multiplexed immunohistochemistry and digital cytometry.
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