Decoding the secret language of photosynthesis UCRiverside NatureComms
PEP assembly was analyzed via blue-native polyacrylamide gel electrophoresis using a NativePAGE Sample Prep kit and a NativePAGE Novex Bis-Tris Gel system with immunoblot
. One hundred milligrams of seedlings grown under the indicated conditions was ground in liquid nitrogen and resuspended in 3 volumes of BN lysis buffer . Protein extracts were divided into two tubes, one for BN-PAGE and the other for SDS-PAGE. For BN-PAGE, protein extracts were mixed with BN sample buffer , and Benzonase nuclease) and incubated for 60 min at room temperature to degrade DNA/RNA and solubilize the PEP complex. Samples were mixed with 0.
Brasil Últimas Notícias, Brasil Manchetes
Similar News:Você também pode ler notícias semelhantes a esta que coletamos de outras fontes de notícias.
Electron cryo-microscopy reveals the structure of the archaeal thread filament - Nature CommunicationsPili are filamentous appendages on the surface of bacteria and archaea, and play roles in multiple processes such as adhesion, motility and horizontal gene transfer. Here, Gaines et al. describe the structure of a new type of pilus, termed ‘thread’, from the model archaeaon Sulfolobus acidocaldarius.
Consulte Mais informação »
Researchers push preprint reviews to improve scientific communicationsMany researchers see promise in preprint reviews—which represent a radical shift in scholarly communication—but the widespread adoption of the practice faces some thorny obstacles.
Consulte Mais informação »
Latest 'Twitter Files' Detail Communications, Payments Between FBI and TwitterThe latest 'Twitter Files' appear to show communications and payments between the FBI and Twitter about disinformation and the Hunter Biden laptop story. See the potential bias and similarities in coverage from newsnation, dcexaminer and reason:
Consulte Mais informação »
Pamela Corante Joins the Motion Picture Association’s Communications TeamCorante will focus on anti-piracy initiatives when arriving at the top entertainment industry lobbying organization in early January.
Consulte Mais informação »
Number of simultaneously acting global change factors affects composition, diversity and productivity of grassland plant communities - Nature CommunicationsMultiple co-acting environmental pressures could affect ecosystems in ways not predictable based on single factors or pairwise combinations. Here, the authors show that the number of global change factors affects productivity, species composition and diversity of grassland plant communities.
Consulte Mais informação »
Biasing the conformation of ELMO2 reveals that myoblast fusion can be exploited to improve muscle regeneration - Nature CommunicationsMyoblast fusion is essential for development of multinucleated myofibers. Here, the authors show that ablation of Elmo 1 and Elmo2 impairs myoblast fusion in mice, and that expression of an open conformation of Elmo2 promotes muscle regeneration and improves the phenotype of Dysferlin-null dystrophic mice.
Consulte Mais informação »