CRISPR technology improves Huntington'sdisease symptoms in models ucsandiego NatureNeuro
Integrated DNA Technologies PrimeTime qPCR assay Mm.PT.58.28953379 was used as an internal housekeeping control.Striatal samples were homogenized in a buffer containing 50 mM Tris-HCl , 150 mM NaCl, 0.1% SDS, 1.0% NP-40, 0.5% sodium deoxycholate and 1% protease inhibitors. Then, 30 μg of proteins were separated in a 4–20% gradient gel and transferred to a nitrocellulose membrane. The membrane was blotted with the following primary antibodies: anti-HTT, 1:1,000, catalog no.
High-resolution anatomical images were acquired by using a three-dimensional T2-weighted fast spin echo sequence with the following parameters: echo time /repetition time = 40/700 ms; resolution = 0.1 × 0.1 × 0.25 mm; echo train length = 4; number of average = 2; and flip angle = 40°.
Brasil Últimas Notícias, Brasil Manchetes
Similar News:Você também pode ler notícias semelhantes a esta que coletamos de outras fontes de notícias.
Activation of the human insulin receptor by non-insulin-related peptides - Nature CommunicationsThe regulation of plasma glucose levels is effected by insulin. Here, the authors reveal atomic detail of how peptides distinct from insulin bind to and activate the insulin receptor, with implications for design of small-molecule insulin mimetics.
Consulte Mais informação »
‘My Baby Was Born At The Bus Stop:’ One Mum’s Story Of Her Surprise Labour'As I looked up, I saw a group of men peering at me. That’s when I realised we were at a bus stop in broad daylight.'
Consulte Mais informação »
Four children critical in hospital while search continues at frozen lakeLocal fire, police and ambulance chiefs told a press conference on Sunday evening that four children were rescued from the water in cardiac arrest and taken to hospital in a critical condition
Consulte Mais informação »
Model Marie Helvin says mastectomy saved her life after breast cancer diagnosisModel Marie Helvin said shared her feelings towards her ‘life-saving’ mastectomy.
Consulte Mais informação »
Driver gets off with £40 fine for crash which left Lamborghini a write offA driver from Waltham, Lincolnshire got off with a £40 fine after a crash which left a £300,000 car good for scrap.
Consulte Mais informação »