Novel Rutgers COVID vaccine may provide long-lasting protection RutgersU
Vero E6 cells were propagated in DMEM containing L-glutamine and 10% FBS to 80% confluency in multiple T75 flasks , and harvested by gentle dissociation of the monolayer with Accutase Cell Detachment Solution following the instructions of the manufacturer . Pooled cells were washed twice in sterile PBS and checked for viability by the Trypan Blue exclusion method.
At 18 h post-seeding, the cells were washed with sterile PBS , and 400 µL of 10-fold dilutions of the virus inoculum, prepared in serum-free DMEM, was added to each well and incubated at 37 °C with gentle rocking of plates every 15 min for 1 h. Then, the virus inoculum was carefully removed, and the infected cells were overlayed with 4 mL/well of 1.6% agarose prepared in DMEM with 4% FBS.
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